- Open Access
Quantifying numerical and spatial reliability of hippocampal and amygdala subdivisions in FreeSurfer
Brain Informatics volume 10, Article number: 9 (2023)
On-going, large-scale neuroimaging initiatives can aid in uncovering neurobiological causes and correlates of poor mental health, disease pathology, and many other important conditions. As projects grow in scale with hundreds, even thousands, of individual participants and scans collected, quantification of brain structures by automated algorithms is becoming the only truly tractable approach. Here, we assessed the spatial and numerical reliability for newly deployed automated segmentation of hippocampal subfields and amygdala nuclei in FreeSurfer 7. In a sample of participants with repeated structural imaging scans (N = 928), we found numerical reliability (as assessed by intraclass correlations, ICCs) was reasonable. Approximately 95% of hippocampal subfields had “excellent” numerical reliability (ICCs ≥ 0.90), while only 67% of amygdala subnuclei met this same threshold. In terms of spatial reliability, 58% of hippocampal subfields and 44% of amygdala subnuclei had Dice coefficients ≥ 0.70. Notably, multiple regions had poor numerical and/or spatial reliability. We also examined correlations between spatial reliability and person-level factors (e.g., participant age; T1 image quality). Both sex and image scan quality were related to variations in spatial reliability metrics. Examined collectively, our work suggests caution should be exercised for a few hippocampal subfields and amygdala nuclei with more variable reliability.
The hippocampus and amygdala are brain regions that play key roles in generating and modulating our responses to emotions and stress [2, 66]; they are subsequently two of the most commonly explored and cited brain regions in research. In fact, a query on PubMed revealed that nearly 84,000 publications within the last 10 years alone referenced the hippocampus or the amygdala . A clear understanding of the structure and function of brain regions supporting a variety of emotion-related processes has implications for both psychological well-being and physical health . For example, both the hippocampus and amygdala show volumetric alterations in different neurodegenerative diseases and various forms of psychopathologies, including Alzheimer’s, Major Depression, Anxiety Disorders, and Autism [9, 31, 77, 87]. Continued study of these subcortical structures could further our knowledge on emotions, memory, decision making, and other processes and may lead to novel intervention strategies for different disorders.
Early studies focused on the hippocampus and amygdala typically examined volumes of these regions using expert manual tracing [ 8, 28, 32, 47, 51, 79, 88]. These approaches were at the time necessary to obtain reliable and valid measures of the size of these key brain areas, but hand-tracing is often exceedingly time intensive. As work in this space has continued, large-scale structural MRI-data sets (Ns from 100 to 1000 subjects) are now commonly available  and work has shifted from manual tracing of regional volumes. Researchers are now able to leverage ever-improving computational algorithms to automatically segment structural images into their component anatomical structures . These approaches represent a scalable and less demanding method to test relations between volumetric measures of these two structures and psychological variables of interest.
A commonly used software suite, FreeSurfer  provides a host of functions for structural MRI processing and analysis, including segmenting subcortical structures. Past work has examined both the validity and reliability of hippocampus and amygdala segmentation in FreeSurfer [41, 49, 57]. One can think of validity as how well an output aligns with “ground-truth” (e.g., comparing FreeSurfer automated amygdala segments to expertly hand-traced volumes), while reliability reflects consistency of outputs (e.g., comparing FreeSurfer automated amygdala from repeated scans of the same person, without consideration of any “ground-truth”). Previous work has found strong reliability for FreeSurfer in terms of hippocampus and amygdala segmentations. Published reports examining test—retest reliability of subcortical volume measures have noted intraclass correlations from FreeSurfer ranging from 0.977 to 0.987 for the hippocampus and 0.806–0.889 for the amygdala [42, 45, 85]. Results considering validity have been more mixed. Work has investigated validity by comparing the spatial and numeric overlap between the volumes produced by FreeSurfer against those produced by expert hand tracing, finding reasonable Dice coefficients for the hippocampus, but lower performance on the amygdala (Hippocampus Dice coefficient = 0.82; Amygdala Dice coefficient = 0.72) [33, 56].
In considering both the hippocampus and amygdala, each of these brain areas are often discussed as unitary structures; however, a large body of basic molecular and cognitive neuroscience research underscores that the hippocampus and amygdala each consist of multiple distinct subregions with different information-processing roles. For example, the hippocampus can be subdivided into the following regions: Dentate Gyrus, critical for pattern separation ; Cornu Ammonis (CA) 3, central to pattern completion ; CA1, important for input integration from CA3 and entorhinal cortex ; and Subiculum, relevant for memory retrieval . Most of the past structural neuroimaging work has combined all these regions, using measures of whole hippocampal volume. This may mean a loss of specificity regarding associations with basic cognitive processes as well as neurobiological alterations seen in different disorders. By examining subcortical structure at a more fine-grain scale, results can be more precisely fit to their root cause and better interpreted considering their theoretical implications.
Responding to this issue, the developers of FreeSurfer have expanded their segmentation methods to include a more granular segmentation of hippocampal subregions . To do this, they combined ultra-high-resolution T1-weighted scans of post-mortem samples with subfields of the hippocampus segmented by hand, to develop an automated algorithm. With this algorithm, there appears to be good numerical reliability and slightly lower spatial reliability for these segments, mirroring the reliability work focusing on the whole hippocampus. Numerical reliability and ICCs are focused on the consistent overall volume size (as indexed by the number of voxels in a region), whereas spatial reliability and the calculation of Dice coefficients assess that the set of voxels classified are the same across both cases. These forms of reliability are typically correlated, but segments could have high numerical reliability but low spatial reliability. In such a case, the same number of voxels are being labelled as a brain region, but the voxels are in fact spatially divergent (and may not be the same brain area). Past work has observed high numerical and moderately high spatial reliability for the hippocampal subfields, reporting ICCs ranging from 0.70 to 0.97 and Dice coefficients ranging from approximately 0.60–0.90 [7, 81]. While we focus on numerical and spatial reliability here, on-going work with manual segmentation procedures continue to develop—that is, different research groups are still working to establish a consensus for how to segment the hippocampus [44, 89].
The amygdala, similarly, has its own subdivisions and the reliability of the automatic segmentation of these subdivisions is still unclear. The FreeSurfer team also expanded their segmentation pipeline to cover a set of subdivisions for the amygdala. The algorithm they employ is trained on manually segmented amygdala nuclei from high-definition 7 Tesla ex-vivo MR images and divides this structure into 9 labelled sub-regions. They applied this segmentation to data sets looking at populations with autism  and those at risk for Alzheimer's disease  finding significant improvements in pathology detection when this more fined grained view of the amygdala was used in the model . However, direct assessment of numerical and spatial reliability for amygdala subdivisions is limited. Quattrini and colleagues (2020) examined these segments in a modest cohort of individuals (total N = 133) and found reasonable reliability for larger subdivisions (> 200 mm3 for the amygdala; > 300 mm3 for the hippocampus). This work, however, aggregated across 17 research sites and multiple MRI vendors, deployed a dated version of the software (FreeSurfer 6.0), and typically acquired repeated imaging scans across weeks and months. Given these limitations, the consistency of these segments for a more conventional, single-site study, is still an open question, and it is still unclear whether this fine-grained separation is consistent in the areas that the algorithm is automatically dividing and outputting. Such gaps are critical to fill given that many groups are using these algorithms for applied purposes and reporting differences between clinical and non-clinical populations [55, 90].
Motivated by these facts, we seek to provide an in-depth examination of reliability, both numerically and spatially, for FreeSurfer derived hippocampal and amygdala subdivisions. We leverage an open-access data set of repeated structural scans consisting of a robust sample size (N = 928 subjects) that provides precise estimates of reliability variables and unprecedently considers multiple scans to obtain these reliability parameters. Specifically, three repeated structural scans were taken on the same day and same scanner for a total of over 2700 included scans; other similar investigations of reliability have relied on much smaller sample sizes, scans repeated across several days, multiple scanners, and outdated neuroimaging software (e.g., . This investigation minimizes interference from different scanners and benefits from a large sample size, three repeated scans, and up-to-date methods to understand reliability.
In addition to this first-order goal of considering reliability, we also wanted to consider whether person-level (e.g., age, sex) and MR-acquisition (e.g., image quality) factors influence the reliability of these subdivisions. Of note, recent work suggests that MR quality can significantly drive signal variations in structural MRI analyses [27, 49]. Pursuing these aims can inform whether all subdivisions are truly “reliable” and should be explored in FreeSurfer-related analyses, or if caution should be taken in morphometric comparisons (especially for those working in applied areas, e.g., tests of amygdala subdivisions in depressed vs. non-depressed groups). It is critical to highlight less reliable segmentations given the popularity of the hippocampus and amygdala in research and the widespread deployment of FreeSurfer software.
Data from an open-access neuroimaging initiative, the Amsterdam Open MRI Collection (AOMIC) , were used to investigate numerical and spatial reliability of FreeSurfer’s amygdala and hippocampal subregion segmentation algorithms. AOMIC includes structural and functional neuroimaging scans from participants, repeating scans in the same session to see the stability of MRI-based metrics. For this work, data from 928 participants (Average Age = 22.08, Standard Deviation = 1.88) were examined. The majority of participants (n = 913, 98% of the sample) had three T1-weighted MR images collected in the same scanning session, while a small subgroup of participants (n = 15, ~ 2% of the sample) had two T1-weighted scans. All repeated MRI scans were acquired with the same imaging parameters (noted below).
2.2 MRI scan parameters
MR images were acquired with a Phillips 3 T Intera scanner at the University of Amsterdam. T1-weighted MR images were acquired using a sagittal 3D-MPRAGE sequence (TR/TE = 8.1 ms/3.7 ms, 1mm3 voxel, matrix size = 64 × 64). Additional details about the scanning parameters are described by Snoek and colleagues (2021). MRI Images were visually inspected to determine if a participant’s scans should be included in subsequent processing steps (e.g., FreeSurfer).
2.3 Structural neuroimaging processing (FreeSurfer)
Standard-processing approaches from FreeSurfer (e.g., cortical reconstruction; volumetric segmentation) were performed in version 7.1 (Stable Release, May 11, 2020) This was implemented via Brainlife (http://io), a free, publicly funded, cloud-computing platform designed for developing reproducible neuroimaging processing pipelines and sharing data [4, 65]. FreeSurfer is a widely documented and freely available morphometric processing tool suite (http://surfer.nmr.mgh.harvard.edu). The technical details of this software suite are described in prior publications [14, 20,21,22,23, 23, 24, 24]. Briefly, this processing includes motion correction and intensity normalization of T1-weighted images, removal of non-brain tissue using a hybrid watershed/surface deformation procedure , automated Talairach transformation, segmentation of the subcortical white matter and deep gray matter volumetric structures (including hippocampus, amygdala, caudate, putamen, ventricles), tessellation of the gray matter white matter boundary, and derivation of cortical thickness. Scans from two subjects failed to run to completion in this pipeline and both subjects were removed from further analysis.
FreeSurfer version 7.1 natively includes options to segment hippocampal subfields and amygdala nuclei. The hippocampal segmentation method  is based on a hippocampal atlas initially produced from a data set of 15 hand-traced high definition ex-vivo T1-weighted 7 T scans then applied to a set of 39 standard resolution in-vivo MPRAGE scans using parameterized mesh deformations and a probabilistic atlas classification approach. This atlas is used for algorithmic segmentation of MR images pre-processed through the FreeSurfer recon-all pipeline. These images were classified using a parameterized generative model and optimizing the likelihood that any given voxel belongs to the label of a particular hippocampal region in a Bayesian inference framework (for Additional file 1, see . The atlas for this method partitions the hippocampus into the following 12 subfields: (1) Parasubiculum, (2) Presubiculum [Head and Body], (3) Subiculum [Head and Body], (4) CA1 [Head and Body], (5) CA3 [Head and Body], (6) CA4 [Head and Body], (7) Granule Cell and Molecular Layer of the Dentate Gyrus [GC-ML-DG, Head and Body], (8) Molecular layer [Head and Body], (9) Fimbria, (10) Hippocampal Fissure, (11) Hippocampal Tail, and (12) Hippocampus-Amygdala-Transition-Area (HATA. This yields nineteen subdivisions from FreeSurfer (including these regions and head/body divisions.
For the amygdala, the automated segmentation method  is based on an atlas produced from 10 hand-traced high definition ex-vivo T1w 7 T scans (5 participants traced bilaterally). As in the hippocampal atlas, this manually segmented ex-vivo data were then applied to the probabilistic classification of the nodes on a parameterized deformation mesh of the amygdala. Similar to the hippocampus, the segmentation of later input data is performed in the framework of Bayesian inference. The amygdala atlas partitions the structure into the following 7 subnuclei: (1) Lateral, (2) Basal, (3) Central, (4) Medial, (5) Cortical, (6) Accessory Basal, (7) Paralaminar. Two additional subdivisions, the Corticoamygdaloid Transition Area and Anterior Amygdaloid Area, are also output.
Of note, here we processed scans from each participant using the “cross-sectional” pipeline. This is in contrast to FreeSurfer’s longitudinal stream that creates an unbiased within-subject template image to improve temporal consistency and reduce potential source of bias (e.g., misregistration) [69, 70]. We consider scans processed using FreeSurfer’s “longitudinal” pipeline in supplemental analyses (see Additional file 1). Cross-sectional pipelines were applied to the three scans for each participant. For both the hippocampal subfields and amygdala nuclei, volume (in mm3 for each subdivision was extracted and used in numerical reliability analysis. Spatial information (labelled voxels in axial, coronal, and spatial orientations was output for each subdivision. Each participant’s T1-weighted scan was then transformed to a common space using FMRIB's Linear Image Registration Tool (degrees of freedom = 6; registering the 2nd and 3rd scans to the participant’s 1st scan). This transformation matrix was then saved and applied to each volume’s labelled output for hippocampal and amygdala subdivisions using a nearest neighbour interpolation; these transformed hippocampal and amygdala subdivisions were then used in spatial reliability analysis.
2.4 Automated MRI image quality assessment
The Computational Anatomy Toolbox 12 (CAT12) toolbox from the Structural Brain Mapping group, implemented in SPM12, was used to generate a quantitative metric indicating the quality of each collected MR image . The method employed considers four summary measures of image quality: (1) noise to contrast ratio, (2) coefficient of joint variation, (3) inhomogeneity to contrast ratio, and (4) root mean squared voxel resolution. To produce a single aggregate metric that serves as an indicator of overall quality, this toolbox normalizes each measure and combines them using a kappa statistic-based framework, for optimizing a generalized linear model through solving least squares . After extracting one quality metric for each scan, we generated three values that represent the difference between two scans (i.e., Scan 1–Scan 2; Scan 1–Scan 3; Scan 2–Scan 3). After taking the absolute value of each of these difference scores, we then averaged them together and used this as a measure of aggregate image quality.
2.5 Derivation of reliability measures
To assess the reliability of numerical volumes output for hippocampus and amygdala subdivisions, we computed intraclass correlation coefficients (ICC) between each labelled sub-region for the test and the retest MRI scans. Of note, an ICC is a descriptive statistic indicating the degree of agreement between two (or more) sets of measurements. The statistic is similar to a bivariate correlation coefficient insofar as it has a range from 0 to 1 and higher values represent a stronger relationship. An ICC, however, differs from the bivariate correlation in that it works on groups of measurements and gives an indication of the numerical cohesion across the given groups . The ICC was calculated separately for each sub-region using the statistical programming language R, with the icc function from the package ‘irr’ . A two-way model with absolute agreement was used to investigate the reliability of subdivision segmentation; this was calculated for each subdivision’s volume (in mm3). Although there are no definitive guidelines for precise interpretation of ICCs, results have frequently been binned into three (or four) quality groups, where 0.0–0.5 is “poor”, 0.50–0.75 is “moderate”, 0.75–0.9 is “good” and 0.9–1.0 is “excellent” [10, 43].
In addition to ICCs, Bland–Altman metrics were calculated for each hippocampal and amygdala subdivision using the function blandr.statistics from the package 'blandr' . In this approach, the mean differences (“bias”) between the FreeSurfer outputs (comparing the first and second scan, the first and third scan, and the second and third scan) were first calculated and presented as a portion of the mean volume. We took the absolute value of each of these three values and averaged them together to represent the average Bland–Altman metric across the three scans for a given brain region. Bland–Altman plots were also constructed for a small number of subdivisions to assess agreement between FreeSurfer outputs.
Although ICCs and Bland–Altman metrics serve as indicators of numerical reliability, these may still be incomplete, particularly when we think about the spatial information present in MRI volumes. Indeed, even with numerical similarity, there may be discrepancies in the specific spatial voxels labelled for a given subdivision. To assess whether the voxels assigned to each region were the same between the two timepoints, we calculated the Sørensen-Dice Coefficient using the @DiceMetric program in the AFNI fMRI software package . The Dice coefficient is calculated as (2TP)/(2TP + FP + FN) [TP = True Positive; FP = False Positive; FN = False Negative] and gives an equal weight to criteria of positive predictive value and sensitivity in assessing spatial reliability of subdivisions. Dice coefficients were averaged across the three scans for each brain region to obtain an overall metric of spatial reliability (e.g., one Dice value for the Left Lateral Nucleus, one Dice value for the Right Lateral Nucleus). As recommended by past reports [91, 92], we considered Dice coefficients ≥ 0.700 as exhibiting “good” spatial overlap.
2.6 Statistical analysis
Once overall reliability metrics were calculated, we examined person-level (e.g., age, sex) and MR-acquisition (e.g., MRI quality) factors in relation to these measures. Many different factors may impact amygdala and hippocampal segmentation. For example, past work suggests volumes (of the hippocampus and amygdala) vary with participant age and sex; this association is particularly strong for the hippocampus [16, 61] and suggestive data similarly for the amygdala [51, 52, 64, 67]. Finally, image quality has been shown to have a significant effect on brain volume measurements . Noisier images may lead to gray/white matter misclassification, and impact reliability between different scans. To consider these potential effects, we examined each region's reliability in relation to age, sex, and difference in the CAT12 quality metric. Of note, the average difference in quality between the three scans (described in Automated MRI Image Quality Assessment) was included in these analyses.
We computed Pearson’s r correlations between Dice coefficients and relevant metrics (i.e., MRI scan quality, sex, and age) using the R function ‘rcorr’ from package Hmisc (Harrell Jr., 2022). Specifically, for each participant we correlated relevant metrics (the difference in scan quality across 3 scans, sex, and age) with the average of the Dice values across 3 scans for each left and right brain region. We highlighted in Tables 3 and 4 correlations with p values smaller, or equal to, 0.05.
3.1 Hippocampus reliability
Using ICC analysis, we found consistently reasonable levels of numerical reliability for hippocampal subfields. Multiple regions demonstrated “excellent” reliability (ICC ≥ 0.90), while all of the subfields were at least in the “good” range (ICC = 0.75–0.90). See Table 1 for values from the 19 subfield segmentations in each hemisphere. Bland—Altman bias indicated some variability with differences between scans, as a portion of that structure’s volume, ranging from 0.078 to 1.198%. See Fig. 1 for a density plot of the average difference in volume estimation across three scans for two hippocampal subfields.
Using Dice coefficients as metrics of spatial reliability, results became a bit more variable with 11 areas showing “excellent” spatial reliability, 7 areas showing “good” spatial reliability, and one area (left Hippocampal fissure) showing poor spatial reliability (Dice coefficient < 0.5). See Fig. 2 for a plot of all Hippocampal Dice coefficient values and Fig. 3 for an example of regions with acceptable spatial reliability (parasubiculum) and poor spatial reliability (hippocampal fissure).
3.2 Amygdala reliability
Within the amygdala, the numerical reliability was “excellent” for about 67% of the regions (ICC > 0.90), while the remainder of the regions were in the “good” range (ICC = 0.75–0.90) (see Table 2). Bland–Altman bias values were somewhat variable with a range of 0.058–1.563%. See Fig. 4 for a density plot of the average difference in volume estimation across three scans for two amygdala subnuclei.
Regarding spatial reliability, seven areas demonstrated excellent or good reliability (> 0.7) including the lateral, basal, and accessory basal subnuclei (See Table 2). There were, however, areas with poor spatial reliability, including the Medial and Paralaminar Nuclei (Dice Coefficients = 0.30–0.4, See Fig. 5 for a plot of all Amygdala Dice Coefficient values). Figure 6 displays a depiction of the Lateral nucleus, an area with acceptable spatial reliability, and the Paralaminar nucleus, an area with poor spatial reliability.
3.3 Reliability differences in relation to person-level and MR-acquisition factors
We next examined associations between spatial reliability and subject-level variables. Correlations between the Hippocampal-subfield Dice coefficients and our subject-level variables are shown in Table 3. Differences in image quality and participant sex were significantly and negatively related to volumes in a majority of the hippocampal subfields at the p < 0.01 level (shown in Table 3). Age was significantly correlated with only a small subset of right hippocampal subfield volumes.
Correlations between spatial reliability and subject-level variables for the amygdala nuclei are reported in Table 4. Image quality was significantly and negatively related to a number of regions including the lateral nucleus, the right basal nucleus, the corticoamygdaloid transition, and the right anterior amygdaloid area (at p < 0.01). The spatial reliability of several regions was also significantly and associated with sex and age.
In this paper, we assessed the numerical and spatial reliability of FreeSurfer’s hippocampal and amygdala subdivision segmentation algorithms. The ICCs, serving as our indicator of numerical reliability, were reasonable (hippocampal subfields: 0.887–0.979; amygdala nuclei: 0.832–0.964), indicating that FreeSurfer is generally numerically reliable in providing overall volume for each subregion. Using Bland–Altman metrics of bias as an additional proxy of numerical reliability suggests a few regions exhibited variability in segmentation across scans; specifically, 5 regions across the hippocampus and amygdala showed ≥ 1% bias in volume from one scan to the next. This is concerning given that individuals with dementia (e.g., Alzheimer's disease) or recurrent mental health issues (e.g., depression) often only differ 1–5% from control groups in subcortical volumes (e.g., [40, 46, 74]. The Dice coefficients, serving as our indicator of spatial reliability, were reasonable, though lower than the ICCs. Of potential concern, a few subdivisions in both the hippocampus and amygdala had fairly low spatial reliability, suggesting unreliable segmentation. Examined collectively, applied researchers should take care when applying these types of automated segmentation techniques, especially if not thoroughly trained in amygdala and hippocampal anatomy.
While our results suggest that many of the volumetric outputs of amygdala and hippocampal subdivisions are mostly numerically reliable, the drop in spatial reliability may mean researchers should exercise caution in the analysis and interpretation of areas with poor spatial reliability. For example, the hippocampal fissure, paralaminar nucleus (amygdala), and medial nucleus (amygdala) showed poor spatial reliability (< 0.5) through their Dice coefficients. Because the spatial reliability of these areas is relatively poor, studies that interpret changes in volume within or across subjects might be using segmentations which contain improperly (or inconsistently) classified voxels within those regions. For example, several studies have already reported significant findings from the paralaminar nucleus of the amygdala [55, 90]; given the questionable reproducibility of its anatomical bounding, these findings may require further verification.
Connected to spatial reliability, there are a few potential drivers of the substandard performance in this domain. First, these areas are small and may be difficult to isolate. In such cases, even a few mislabelled voxels can greatly influence spatial overlap. Many of the areas with the lowest spatial reliability are also the smallest subdivisions. For example, the paralaminar and the medial nuclei of the amygdala range between 20 and 60 mm3 in our sample and have some of the lowest spatial reliability values. However, this is not the only factor hampering performance, as other structures (of similar sizes) have reasonable spatial reliability values (e.g., HATA ≥ 0.760 Dice coefficients; Parasubiculum ≥ 0.710 Dice coefficients), while comparatively larger structures (e.g., hippocampal-fissure; CA3-body) demonstrate lower spatial reliability. Second, irregular MR contrast is often common to these areas, especially for the amygdala. Given the close vicinity to bone and sinuses, there is typically susceptibility-related dropout, field inhomogeneities, and physiological artifacts in the amygdala and the hippocampus [54, 71, 82]. This may introduce inconsistent gray/white matter contrast, complicating isolation of different subdivisions. Finally, several amygdala and hippocampal subdivisions are irregularly and complexly shaped. For example, both the anterior and posterior borders of the amygdala are difficult to consistently demarcate [1, 11, 19, 80]. Many past reports using manual tracing actually employ “heuristics” rather than clear anatomical boundaries (e.g.,  used a “semicircle substitution”).
Given these challenges, it is critical to advance novel approaches to segment the hippocampus and amygdala into subdivisions while still maintaining high validity and reproducibility. In regard to the hippocampus, there has been a great deal of progress made by the Hippocampal Subfields Group (HSG); this is a collaboration of > 200 imaging and anatomy experts worldwide that has established guidelines for appropriate MRI acquisition for researchers interested in the hippocampus, as well as developing candidate protocols for the segmentation of hippocampal subregions (eg., [62, 84, 89]. This and other related work have suggested important ways to validate automatic segmentation, including not only comparison to manual delineations, but also replicating known disease effects (e.g., . The HSG and FreeSurfer protocols provide differing guidance on how to subdivide the hippocampus (see Table 3 of  for HSG subdivisions). Comparison between HSG and FreeSurfer subdivisions reveals that some hippocampal regions are divided with more granularity according to HSG guidelines, while some regions are more granular with FreeSurfer. For example, while FreeSurfer divides the subiculum, presubiculum, CA1, CA3, CA4, and other regions into “head” and “body,” the HSG does not. Our work suggests that some of these smaller subdivisions may be less reliable than others; Dice coefficients for CA3 head and CA3 body, for instance, were some of the few hippocampal regions in the “good” (and not “excellent”) range. The HSG also includes regions not evaluated by FreeSurfer, including the Entorhinal Cortex, Parahippocampal Cortex, Perirhinal Cortex, while Freesurfer includes regions not considered by the HSG. Importantly, one of the areas identified by our work as having poor reliability—the hippocampal fissure—is not listed as an HSG subdivision. Based on these considerations, we echo HSG’s call to harmonize across protocols, especially across regions with less reliability (e.g., hippocampal fissure), and to re-evaluate some FreeSurfer subdivisions.
Similar joint efforts to HSG are not, to our knowledge, currently underway for amygdala subnuclei segmentation. Convening such a collaborative could be particularly impactful moving forward, especially as debate has been fairly continuous regarding subdivisions of the amygdala at the histological level (e.g., . Our results found that, across both the hippocampus and amygdala, most regions found to have less satisfactory reliability were in the amygdala. Given the popularity of the amygdala as a brain region (e.g., recent reports have found that manuscripts findings on the amygdala are more likely to be published in high-impact journals;  and widespread deployment of FreeSurfer by basic and applied researchers, we recommend caution in interpreting findings regarding amygdala subnuclei.
In the interim or the absence of joint efforts to establish more reliable amygdala segmentations, we have several suggestions for higher quality research. It may be reasonable to only consider more macro-level amygdala segmentation (e.g., basolateral, centromedial, basomedial, and amygdaloid cortical complexes, as detailed by . Many groups have moved towards this idea, aggregating subdivisions using latent factor modelling and other techniques to group related regions (e.g., . There is, however, ongoing debate about specific best practices, as even established guidelines for MRI acquisition or landmark in in-vivo data may present additional unforeseen challenges (e.g., Special hippocampal acquisitions providing incomplete coverage of target structure; In-vivo MRI does not supply enough features to define many hippocampal subfield boundaries). In addition, findings suggest that magnetic resonance images with 1 × 1 × 1 1 mm3 resolution are too low in quality for investigations of hippocampal subfields ; future work should, therefore, strive to use higher resolution images with FreeSurfer segmentation (i.e., resolution smaller than 1 × 1 × 1 mm3). Finally, single modality structural imaging (e.g., a T1 scan without a T2 scan) is likely less reliable; protocols could require routines to have multiple imaging modalities or restrict output to more reliable regions if input contains a single imaging modality.
As noted in our introduction, our findings only speak to the reliability of these measures, and not the validity of these segments. Investigations of validity require comparison of automated output with “ground-truth” data typically derived from hand-tracing. Given that our data set contains over 2700 scans (and, therefore, the same number of amygdalae that would require tracing), such an endeavor is less practical for this particular sample. Future work should establish the validity of these FreeSurfer subnuclei divisions, particularly considering the popularity of the amygdala as a brain region  and FreeSurfer as a segmentation software. Previous work has compared FreeSurfer’s hippocampal subfields to hand drawn volumes [35, 86]; however, there is yet to be any comparison of automated amygdala subnuclei segmentation to hand-tracing. Reliable methods exist for expert manual segmentation of the amygdala [3, 19, 36]; however, this typically requires high-resolution and high-field strength neuroimaging (i.e., > 3 T MRI Scanner, sub-millimeter voxels). Studies looking at the degree of overlap between such methods and the FreeSurfer algorithm for amygdala segmentation would be helpful for effective evaluation of validity.
Our goal was to present reliability analyses that would be the most relevant to the ‘typical’ structural study, where a T1-weighted single scan is acquired for each participant, recruited from a specific local area. In considering this approach, it is important to note a few potential limitations of our work. First, we processed test—retest MRI images using the cross-sectional FreeSurfer pipeline; we report in our Supplementary Materials using the longitudinal stream. Results were largely consistent across the two pipelines, with several hippocampal subfields and amygdala subnuclei demonstrating decreased numeric reliability with the longitudinal processing stream. Furthermore, the following regions were consistently highlighted as having less-than-excellent spatial reliability across both streams: in the hippocampus, the hippocampal fissure, and in the amygdala, the central, cortical, paralaminar, and medial nuclei. We present results from the cross-sectional processing pipeline, as is done in other studies of reliability (e.g., , because FreeSurfer’s longitudinal pipeline is not independently segmenting the different MRI scans. This violates some theoretical aspects of test—retest reliability and would be expected to produce a more favorable set of reliability metrics for FreeSurfer’s methods.
Second, we only used T1-weighted scans in FreeSurfer, but additional MRI volumes (e.g., T2-weighted) from the same subjects may yield a more reliable segmentation. FreeSurfer’s developers have worked to allow the amygdala and hippocampal subdivision routines to accept high-resolution T2-weighted volumes, and this should be investigated in future work. Third, the sample is a rather homogenous group of individuals and may not represent the greater population. All participants were recruited from the University of Amsterdam, with limited racial and ethnic variability. Similarly, all participants were neurotypical young adults in a constrained age range (Mean = 22.08 ± 1.88). Additional work considering reliability of the method in a diverse set of populations (e.g., pediatric, elderly, mild cognitive impairment) would be helpful in ascertaining how well these findings generalize outside of our sample population. Finally, this analysis focused on the reliability of FreeSurfer 7.1, since this reflects a more current version of the software. Future work could consider comparing segmentations derived from current FreeSurfer versions with past versions of this software to facilitate our understanding across extant and emerging literature. Recently published work reflects an analogous endeavor for non-segmented brain regions (e.g., the whole amygdala), highlighting key anatomical areas that were less compatible across FreeSurfer software versions 5.3, 6.0, and 7.1 . These authors found good-to-excellent reliability across software versions for subcortical regions (including the hippocampus and amygdala) and reported that FreeSurfer version 7.1 was generally advantageous over earlier versions.
Limitations notwithstanding, our work extends the information provided by previous publications regarding the reliability of FreeSurfer’s subcortical segmentation for the hippocampus, amygdala, and their respective subregions. To our knowledge, this is the first work to directly investigate the test—retest reliability of the amygdala nuclei algorithm in FreeSurfer 7. The strengths of our work include a large sample size, the use of FreeSurfer’s more robust longitudinal pipeline, and the report of mathematically rigorous measures of reliability. Our work provides additional confidence in interpreting those regions with high reliability and a necessary caution in interpretation of those with poorer results.
Availability of data and materials
Neuroimaging data used in our analyses were sourced from the Amsterdam Open MRI Collection (AOMIC, . FreeSurfer software is publicly and freely available from the FreeSurferWiki resource (http://surfer.nmr.mgh.harvard.edu/fswiki/FreeSurferWiki), which is developed and maintained at the Martinos Center for Biomedical Imaging (http://www.nmr.mgh.harvard.edu/martinos/noFlashHome.php). This software, information and support are provided online at the FreeSurferWiki webpage.
Achten E, Deblaere K, De Wagter C, Van Damme F, Boon P, De Reuck J, Kunnen M (1998) Intra- and interobserver variability of MRI-based volume measurements of the hippocampus and amygdala using the manual ray-tracing method. Neuroradiology 40(9):558–566. https://doi.org/10.1007/s002340050644
Adolphs R (2010) What does the amygdala contribute to social cognition? Ann N Y Acad Sci 1191(1):42–61
Aghamohammadi-Sereshki A, Huang Y, Olsen F, Malykhin NV (2018) In vivo quantification of amygdala subnuclei using 4.7 T fast spin echo imaging. Neuroimage 170:151–163. https://doi.org/10.1016/j.neuroimage.2017.03.016
Avesani P, McPherson B, Hayashi S, Caiafa CF, Henschel R, Garyfallidis E, Kitchell L, Bullock D, Patterson A, Olivetti E, Sporns O, Saykin AJ, Wang L, Dinov I, Hancock D, Caron B, Qian Y, Pestilli F (2019) The open diffusion data derivatives, brain data upcycling via integrated publishing of derivatives and reproducible open cloud services. Sci Data 6(1):69. https://doi.org/10.1038/s41597-019-0073-y
Behrens TEJ, Fox P, Laird A, Smith SM (2013) What is the most interesting part of the brain? Trends Cogn Sci 17(1):2–4. https://doi.org/10.1016/j.tics.2012.10.010
Bittner KC, Grienberger C, Vaidya SP, Milstein AD, Macklin JJ, Suh J, Tonegawa S, Magee JC (2015) Conjunctive input processing drives feature selectivity in hippocampal CA1 neurons. Nat Neurosci 18(8):1133–1142. https://doi.org/10.1038/nn.4062
Brown EM, Pierce ME, Clark DC, Fischl BR, Iglesias JE, Milberg WP, McGlinchey RE, Salat DH (2020) Test-retest reliability of FreeSurfer automated hippocampal subfield segmentation within and across scanners. Neuroimage 210:116563. https://doi.org/10.1016/j.neuroimage.2020.116563
Caldwell JZK, Armstrong JM, Hanson JL, Sutterer MJ, Stodola DE, Koenigs M, Kalin NH, Essex MJ, Davidson RJ (2015) Preschool externalizing behavior predicts gender-specific variation in adolescent neural structure. PLoS ONE 10(2):e0117453. https://doi.org/10.1371/journal.pone.0117453
Campbell S, Marriott M, Nahmias C, MacQueen GM (2004) Lower hippocampal volume in patients suffering from depression: a meta-analysis. Am J Psychiatry 161(4):598–607. https://doi.org/10.1176/appi.ajp.161.4.598
Cicchetti DV (1994) Guidelines, criteria, and rules of thumb for evaluating normed and standardized assessment instruments in psychology. Psychol Assess 6(4):284–290
Convit A (1999) MRI volume of the amygdala: a reliable method allowing separation from the hippocampal formation. Psychiatry Res Neuroimaging 90(2):113–123. https://doi.org/10.1016/S0925-4927(99)00007-4
Cox RW (1996) AFNI: software for analysis and visualization of functional magnetic resonance neuroimages. Comput Biomed Res 29(3):162–173. https://doi.org/10.1006/cbmr.1996.0014
Dahnke, R., Ziegler, G., Grosskreutz, J., Gaser, C. (2015). Quality Assurance in Structural MRI. https://doi.org/10.1314/RG.2.2.16267.44321
Dale AM, Fischl B, Sereno MI (1999) Cortical surface-based analysis. Neuroimage 9(2):179–194
Datta D (2017) blandr: a bland-altman method comparison package for r. zenodo. Ann Clin Biochem Int J Laboratory Med 52(3):11. https://doi.org/10.5281/zenodo.824514
Daugherty AM, Bender AR, Raz N, Ofen N (2016) Age differences in hippocampal subfield volumes from childhood to late adulthood: Lifespan Hippocampal Subfield Volumes. Hippocampus 26(2):220–228. https://doi.org/10.1002/hipo.22517
DeSteno D, Gross JJ, Kubzansky L (2013) Affective science and health: the importance of emotion and emotion regulation. Health Psychol 32(5):474–486. https://doi.org/10.1037/a0030259
Di Martino A, Yan C-G, Li Q, Denio E, Castellanos FX, Alaerts K, Anderson JS, Assaf M, Bookheimer SY, Dapretto M, Deen B, Delmonte S, Dinstein I, Ertl-Wagner B, Fair DA, Gallagher L, Kennedy DP, Keown CL, Keysers C, Milham MP (2014) The autism brain imaging data exchange: towards a large-scale evaluation of the intrinsic brain architecture in autism. Mole Psychiatry 19(6):659–667. https://doi.org/10.1038/mp.2013.78
Entis JJ, Doerga P, Barrett LF, Dickerson BC (2012) A reliable protocol for the manual segmentation of the human amygdala and its subregions using ultra-high resolution MRI. Neuroimage 60(2):1226–1235. https://doi.org/10.1016/j.neuroimage.2011.12.073
Fischl B (2012) FreeSurfer. Neuroimage 62(2):774–781. https://doi.org/10.1016/j.neuroimage.2012.01.021
Fischl B, Salat DH, Busa E, Albert M, Dieterich M, Haselgrove C, van der Kouwe A, Killiany R, Kennedy D, Klaveness S, Montillo A, Makris N, Rosen B, Dale AM (2002) Whole brain segmentation. Neuron 33(3):341–355. https://doi.org/10.1016/S0896-6273(02)00569-X
Fischl B, Salat DH, van der Kouwe AJW, Makris N, Ségonne F, Quinn BT, Dale AM (2004) Sequence-independent segmentation of magnetic resonance images. Neuroimage 23:S69–S84. https://doi.org/10.1016/j.neuroimage.2004.07.016
Fischl B, Sereno MI, Dale AM (1999) Cortical surface-based analysis. Neuroimage 9(2):195–207
Fischl B, Sereno MI, Tootell RBH, Dale AM (1999) High-resolution intersubject averaging and a coordinate system for the cortical surface. Hum Brain Mapp 8(4):272–284
Gamer, M., & Lemon, J. (2012). Package “irr.” 32.
Gaser C, Kurth F (2017) Manual computational anatomy toolbox-CAT12. University of Jena, Structural Brain Mapping Group at the Departments of Psychiatry and Neurology, p 69
Gilmore AD, Buser NJ, Hanson JL (2021) Variations in structural MRI quality significantly impact commonly used measures of brain anatomy. Brain Informatics 8(1):1–15. https://doi.org/10.1186/s40708-021-00128-2
Gunten A, Fox N, Cipolotti L, Ron MA (2000) A volumetric study of hippocampus and amygdala in depressed patients with subjective memory problems. J Neuropsychiatry Clin Neuropsychiatry 12(4):6. https://doi.org/10.1001/jama.1992.03490110111047
Guzman SJ, Schlogl A, Frotscher M, Jonas P (2016) Synaptic mechanisms of pattern completion in the hippocampal CA3 network. Science 353(6304):1117–1123. https://doi.org/10.1126/science.aaf1836
Haddad E, Pizzagalli F, Zhu AH, Bhatt RR, Islam T, Ba Gari I, Dixon D, Thomopoulos SI, Thompson PM, Jahanshad N (2023) Multisite test–retest reliability and compatibility of brain metrics derived from FreeSurfer versions 7.1, 6.0, and 5.3. Hum Brain Mapp 44(4):1515–1532. https://doi.org/10.1002/hbm.26147
Hamilton JP, Siemer M, Gotlib IH (2008) Amygdala volume in major depressive disorder: a meta-analysis of magnetic resonance imaging studies. Mol Psychiatry 13(11):993–1000. https://doi.org/10.1038/mp.2008.57
Hanson JL, Nacewicz BM, Sutterer MJ, Cayo AA, Schaefer SM, Rudolph KD, Shirtcliff EA, Pollak SD, Davidson RJ (2015) Behavioral problems after early life stress: contributions of the hippocampus and amygdala. Biol Psychiat 77(4):314–323. https://doi.org/10.1016/j.biopsych.2014.04.020
Hanson JL, Suh JW, Nacewicz BM, Sutterer MJ, Cayo AA, Stodola DE, Burghy CA, Wang H, Avants BB, Yushkevich PA, Essex MJ, Pollak SD, Davidson RJ (2012) Robust automated amygdala segmentation via multi-atlas diffeomorphic registration. Front Neurosci. https://doi.org/10.3389/fnins.2012.00166
Harrell Jr., F. E. (2022). Hmisc: Harrell Miscellaneous (4.7–2). https://CRAN.R-project.org/package=Hmisc
Herten A, Konrad K, Krinzinger H, Seitz J, von Polier GG (2019) Accuracy and bias of automatic hippocampal segmentation in children and adolescents. Brain Struct Funct 224(2):795–810. https://doi.org/10.1007/s00429-018-1802-2
Hrybouski S, Aghamohammadi-Sereshki A, Madan CR, Shafer AT, Baron CA, Seres P, Beaulieu C, Olsen F, Malykhin NV (2016) Amygdala subnuclei response and connectivity during emotional processing. Neuroimage 133:98–110. https://doi.org/10.1016/j.neuroimage.2016.02.056
Iglesias JE, Augustinack JC, Nguyen K, Player CM, Player A, Wright M, Roy N, Frosch MP, McKee AC, Wald LL, Fischl B, Van Leemput K (2015) A computational atlas of the hippocampal formation using ex vivo, ultra-high resolution MRI: application to adaptive segmentation of in vivo MRI. Neuroimage 115:117–137. https://doi.org/10.1016/j.neuroimage.2015.04.042
Iglesias JE, Van Leemput K, Augustinack J, Insausti R, Fischl B, Reuter M (2016) Bayesian longitudinal segmentation of hippocampal substructures in brain MRI using subject-specific atlases. Neuroimage 141:542–555. https://doi.org/10.1016/j.neuroimage.2016.07.020
Jack CR, Bernstein MA, Fox NC, Thompson P, Alexander G, Harvey D, Borowski B, Britson PJ, Whitwell JL, Ward C, Dale AM, Felmlee JP, Gunter JL, Hill DLG, Killiany R, Schuff N, Fox-Bosetti S, Lin C, Studholme C (2008) The Alzheimer’s disease neuroimaging initiative (ADNI): MRI methods. J Magn Reson Imaging 27(4):685–691. https://doi.org/10.1002/jmri.21049
Jack CR, Shiung MM, Weigand SD, O’Brien PC, Gunter JL, Boeve BF, Knopman DS, Smith GE, Ivnik RJ, Tangalos EG, Petersen RC (2005) Brain atrophy rates predict subsequent clinical conversion in normal elderly and amnestic MCI. Neurology 65(8):1227–1231. https://doi.org/10.1212/01.wnl.0000180958.22678.91
Jovicich J, Czanner S, Han X, Salat D, van der Kouwe A, Quinn B, Pacheco J, Albert M, Killiany R, Blacker D (2009) MRI-derived measurements of human subcortical, ventricular and intracranial brain volumes: Reliability effects of scan sessions, acquisition sequences, data analyses, scanner upgrade, scanner vendors and field strengths. Neuroimage 46(1):177–192. https://doi.org/10.1016/j.neuroimage.2009.02.010
Jovicich J, Marizzoni M, Sala-Llonch R, Bosch B, Bartrés-Faz D, Arnold J, Benninghoff J, Wiltfang J, Roccatagliata L, Nobili F, Hensch T, Tränkner A, Schönknecht P, Leroy M, Lopes R, Bordet R, Chanoine V, Ranjeva J-P, Didic M, Frisoni GB (2013) Brain morphometry reproducibility in multi-center 3T MRI studies: a comparison of cross-sectional and longitudinal segmentations. Neuroimage 83:472–484. https://doi.org/10.1016/j.neuroimage.2013.05.007
Koo TK, Li MY (2016) A Guideline of selecting and reporting intraclass correlation coefficients for reliability research. J Chiropr Med 15(2):155–163. https://doi.org/10.1016/j.jcm.2016.02.012
La Joie R, Olsen R, Berron D, Amunts K, Augustinack J, Bakker A, Bender A, Boccardi M, Bocchetta M, Chakravarty MM, Chetelat G, de Flores R, DeKraker J, Ding S, Insausti R, Kedo O, Mueller SG, Ofen N, Palombo D, Daugherty AM (2020) The development of a valid, reliable, harmonized segmentation protocol for hippocampal subfields and medial temporal lobe cortices: a progress update: neuroimaging/new imaging methods. Alzheimer’s Dementia. https://doi.org/10.1002/alz.046652
Liem F, Mérillat S, Bezzola L, Hirsiger S, Philipp M, Madhyastha T, Jäncke L (2015) Reliability and statistical power analysis of cortical and subcortical FreeSurfer metrics in a large sample of healthy elderly. Neuroimage 108:95–109. https://doi.org/10.1016/j.neuroimage.2014.12.035
Logue MW, van Rooij SJH, Dennis EL, Davis SL, Hayes JP, Stevens JS, Densmore M, Haswell CC, Ipser J, Koch SBJ, Korgaonkar M, Lebois LAM, Peverill M, Baker JT, Boedhoe PSW, Frijling JL, Gruber SA, Harpaz-Rotem I, Jahanshad N, Morey RA (2018) Smaller hippocampal volume in posttraumatic stress disorder: a multisite ENIGMA-PGC Study: subcortical volumetry results from posttraumatic stress disorder consortia. Biol Psychiatry 83(3):244–253. https://doi.org/10.1016/j.biopsych.2017.09.006
MacQueen GM, Campbell S, McEwen BS, Macdonald K, Amano S, Joffe RT, Nahmias C, Young LT (2003) Course of illness, hippocampal function, and hippocampal volume in major depression. PNAS 100(3):1387–1392
Madan CR (2022) Scan once, analyse many: using large open-access neuroimaging datasets to understand the brain. Neuroinformatics 20(1):109–137. https://doi.org/10.1007/s12021-021-09519-6
Madan CR, Kensinger EA (2017) Test–retest reliability of brain morphology estimates. Brain Informatics 4(2):107–121. https://doi.org/10.1007/s40708-016-0060-4
Mai JK, Majtanik M, Paxinos G (2015) Atlas of the Human Brain. Academic Press
Malykhin NV, Bouchard TP, Camicioli R, Coupland NJ (2008) Aging hippocampus and amygdala. NeuroReport 19(5):543–547. https://doi.org/10.1097/WNR.0b013e3282f8b18c
Marwha D, Halari M, Eliot L (2017) Meta-analysis reveals a lack of sexual dimorphism in human amygdala volume. Neuroimage 147:282–294. https://doi.org/10.1016/j.neuroimage.2016.12.021
McGraw KO, Wong SP (1996) Forming inferences about some intraclass correlation coefficients. Psychol Methods 1(1):30–46
Merboldt K-D, Fransson P, Bruhn H, Frahm J (2001) Functional MRI of the Human Amygdala? Neuroimage 14(2):253–257. https://doi.org/10.1006/nimg.2001.0802
Morey RA, Clarke EK, Haswell CC, Phillips RD, Clausen AN, Mufford MS, Saygin Z, Wagner HR, LaBar KS, Brancu M, Beckham JC, Calhoun PS, Dedert E, Elbogen EB, Fairbank JA, Hurley RA, Kilts JD, Kimbrel NA, Kirby A, Yoash-Gantz RE (2020) Amygdala nuclei volume and shape in military veterans with posttraumatic stress disorder. Biol Psychiatry Cognit Neurosci Neuroimaging 5(3):281–290. https://doi.org/10.1016/j.bpsc.2019.11.016
Morey RA, Petty CM, Xu Y, Pannu Hayes J, Wagner HR, Lewis DV, LaBar KS, Styner M, McCarthy G (2009) A comparison of automated segmentation and manual tracing for quantifying hippocampal and amygdala volumes. Neuroimage 45(3):855–866. https://doi.org/10.1016/j.neuroimage.2008.12.033
Morey RA, Selgrade ES, Wagner HR, Huettel SA, Wang L, McCarthy G (2010) Scan-rescan reliability of subcortical brain volumes derived from automated segmentation. Human Brain Mapping, NA-NA. https://doi.org/10.1002/hbm.20973
Mueller SG, Yushkevich PA, Das S, Wang L, Van Leemput K, Iglesias JE, Alpert K, Mezher A, Ng P, Paz K, Weiner MW (2018) Systematic comparison of different techniques to measure hippocampal subfield volumes in ADNI2. NeuroImage Clin 17:1006–1018. https://doi.org/10.1016/j.nicl.2017.12.036
Nacewicz BM, Dalton KM, Johnstone T, Long MT, McAuliff EM, Oakes TR, Alexander AL, Davidson RJ (2006) Amygdala volume and nonverbal social impairment in adolescent and adult males with autism. Arch Gen Psychiatry 63(12):1417–1428. https://doi.org/10.1001/archpsyc.63.12.1417
Neunuebel JP, Knierim JJ (2014) CA3 retrieves coherent representations from degraded input: direct evidence for CA3 pattern completion and dentate gyrus pattern separation. Neuron 81(2):416–427. https://doi.org/10.1016/j.neuron.2013.11.017
Nobis L, Manohar SG, Smith SM, Alfaro-Almagro F, Jenkinson M, Mackay CE, Husain M (2019) Hippocampal volume across age: nomograms derived from over 19,700 people in UK Biobank. NeuroImage Clin 23:101904. https://doi.org/10.1016/j.nicl.2019.101904
Olsen RK, Carr VA, Daugherty AM, La Joie R, Amaral RSC, Amunts K, Augustinack JC, Bakker A, Bender AR, Berron D, Boccardi M, Bocchetta M, Burggren AC, Chakravarty MM, Chételat G, Flores R, DeKraker J, Ding S, Geerlings MI (2019) Progress update from the hippocampal subfields group. Alzheimer’s Dementia Diagnosis Assess Disease Monitoring 11(1):439–449. https://doi.org/10.1016/j.dadm.2019.04.001
Oshri A, Gray JC, Owens MM, Liu S, Duprey EB, Sweet LH, MacKillop J (2019) Adverse childhood experiences and amygdalar reduction: high-resolution segmentation reveals associations with subnuclei and psychiatric outcomes. Child Maltreat 24(4):400–410. https://doi.org/10.1177/1077559519839491
Perlaki G, Orsi G, Plozer E, Altbacker A, Darnai G, Nagy SA, Horvath R, Toth A, Doczi T, Kovacs N, Bogner P, Schwarcz A, Janszky J (2014) Are there any gender differences in the hippocampus volume after head-size correction? A volumetric and voxel-based morphometric study. Neurosci Lett 570:119–123. https://doi.org/10.1016/j.neulet.2014.04.013
Pestilli F (2018) Human white matter and knowledge representation. PLOS Biol 16(4):e2005758. https://doi.org/10.1371/journal.pbio.2005758
Phelps EA (2004) Human emotion and memory: interactions of the amygdala and hippocampal complex. Curr Opin Neurobiol 14(2):198–202. https://doi.org/10.1016/j.conb.2004.03.015
Pressman PS, Noniyeva Y, Bott N, Dutt S, Sturm V, Miller BL, Kramer JH (2016) Comparing volume loss in neuroanatomical regions of emotion versus regions of cognition in healthy aging. PLoS ONE 11(8):e0158187. https://doi.org/10.1371/journal.pone.0158187
Quattrini G, Pievani M, Jovicich J, Aiello M, Bargalló N, Barkhof F, Bartres-Faz D, Beltramello A, Pizzini FB, Blin O, Bordet R, Caulo M, Constantinides M, Didic M, Drevelegas A, Ferretti A, Fiedler U, Floridi P, Gros-Dagnac H, Marizzoni M (2020) Amygdalar nuclei and hippocampal subfields on MRI: test-retest reliability of automated volumetry across different MRI sites and vendors. Neuroimage. https://doi.org/10.1016/j.neuroimage.2020.116932
Reuter M, Rosas HD, Fischl B (2010) Highly accurate inverse consistent registration: a robust approach. Neuroimage 53(4):1181–1196. https://doi.org/10.1016/j.neuroimage.2010.07.020
Reuter M, Schmansky NJ, Rosas HD, Fischl B (2012) Within-subject template estimation for unbiased longitudinal image analysis. Neuroimage 61(4):1402–1418. https://doi.org/10.1016/j.neuroimage.2012.02.084
Robinson S, Windischberger C, Rauscher A, Moser E (2004) Optimized 3 T EPI of the amygdalae. Neuroimage 22(1):203–210. https://doi.org/10.1016/j.neuroimage.2003.12.048
Roy DS, Kitamura T, Okuyama T, Ogawa SK, Sun C, Obata Y, Yoshiki A, Tonegawa S (2017) Distinct neural circuits for the formation and retrieval of episodic memories. Cell 170(5):1000–1012. https://doi.org/10.1016/j.cell.2017.07.013
Saygin ZM, Kliemann D, Iglesias JE, van der Kouwe AJW, Boyd E, Reuter M, Stevens A, Van Leemput K, McKee A, Frosch MP, Fischl B, Augustinack JC (2017) High-resolution magnetic resonance imaging reveals nuclei of the human amygdala: manual segmentation to automatic atlas. Neuroimage 155:370–382. https://doi.org/10.1016/j.neuroimage.2017.04.046
Schmaal L, Veltman D, Erp T, Samann P, Frodl T (2016) Subcortical brain alterations in major depressive disorder: findings from the ENIGMA major depressive disorder working group. Mol Psychiatry 21(6):806–812
Ségonne F, Dale AM, Busa E, Glessner M, Salat D, Hahn HK, Fischl B (2004) A hybrid approach to the skull stripping problem in MRI. Neuroimage 22(3):1060–1075. https://doi.org/10.1016/j.neuroimage.2004.03.032
Snoek L, van der Miesen MM, Beemsterboer T, van der Leij A, Eigenhuis A, Steven Scholte H (2021) The Amsterdam open MRI collection, a set of multimodal MRI datasets for individual difference analyses. Scientific Data 8(1):85. https://doi.org/10.1038/s41597-021-00870-6
Stanfield AC, McIntosh AM, Spencer MD, Philip R, Gaur S, Lawrie SM (2008) Towards a neuroanatomy of autism: a systematic review and meta-analysis of structural magnetic resonance imaging studies. Eur Psychiatry 23(4):289–299. https://doi.org/10.1016/j.eurpsy.2007.05.006
Swanson LW, Petrovich GD (1998) What is the amygdala? Trends Neurosci 21(8):323–331. https://doi.org/10.1016/S0166-2236(98)01265-X
von Gunten A, Ron MA (2004) Hippocampal volume and subjective memory impairment in depressed patients. Eur Psychiatry 19(7):438–440. https://doi.org/10.1016/j.eurpsy.2004.05.003
Watson C, Andermann F, Gloor PMDP, Jones-Gotman M, Peters T, Evans A, Leroux G (1992) Anatomic basis of amygdaloid and hippocampal volume measurement by magnetic resonance imaging. Neurology 42(9):1743–1750
Whelan CD, Hibar DP, van Velzen LS, Zannas AS, Carrillo-Roa T, McMahon K, Prasad G, Kelly S, Faskowitz J, deZubiracay G, Iglesias JE, van Erp TGM, Frodl T, Martin NG, Wright MJ, Jahanshad N, Schmaal L, Sämann PG, Thompson PM (2016) Heritability and reliability of automatically segmented human hippocampal formation subregions. Neuroimage 128:125–137. https://doi.org/10.1016/j.neuroimage.2015.12.039
Windischberger C, Langenberger H, Sycha T, Tschernko EM, Fuchsjäger-Mayerl G, Schmetterer L, Moser E (2002) On the origin of respiratory artifacts in BOLD-EPI of the human brain. Magn Reson Imaging 20(8):575–582. https://doi.org/10.1016/S0730-725X(02)00563-5
Wisse LEM, Chételat G, Daugherty AM, de Flores R, la Joie R, Mueller SG, Stark CEL, Wang L, Yushkevich PA, Berron D, Raz N, Bakker A, Olsen RK, Carr VA (2021) Hippocampal subfield volumetry from structural isotropic 1 mm3 MRI scans: a note of caution. Hum Brain Mapp 42(2):539–550. https://doi.org/10.1002/hbm.25234
Wisse LEM, Daugherty AM, Olsen RK, Berron D, Carr VA, Stark CEL, Amaral RSC, Amunts K, Augustinack JC, Bender AR, Bernstein JD, Boccardi M, Bocchetta M, Burggren A, Chakravarty MM, Chupin M, Ekstrom A, de Flores R, Insausti R (2017) A harmonized segmentation protocol for hippocampal and parahippocampal subregions: Why do we need one and what are the key goals?: a harmonized hippocampal subfield protocol: key goals and impact. Hippocampus 27(1):3–11. https://doi.org/10.1002/hipo.22671
Wonderlick J, Ziegler D, Hosseinivarnamkhasti P, Locascio J, Bakkour A, Vanderkouwe A, Triantafyllou C, Corkin S, Dickerson B (2009) Reliability of MRI-derived cortical and subcortical morphometric measures: effects of pulse sequence, voxel geometry, and parallel imaging. Neuroimage 44(4):1324–1333. https://doi.org/10.1016/j.neuroimage.2008.10.037
Worker A, Dima D, Combes A, Crum WR, Streffer J, Einstein S, Mehta MA, Barker GJ, Williams SCR, O’daly O (2018) Test–retest reliability and longitudinal analysis of automated hippocampal subregion volumes in healthy ageing and Alzheimer’s disease populations. Hum Brain Mapp 39(4):1743–1754. https://doi.org/10.1002/hbm.23948
Yang J, Pan P, Song W, Huang R, Li J, Chen K, Gong Q, Zhong J, Shi H, Shang H (2012) Voxelwise meta-analysis of gray matter anomalies in Alzheimer’s disease and mild cognitive impairment using anatomic likelihood estimation. J Neurol Sci 316(1–2):21–29. https://doi.org/10.1016/j.jns.2012.02.010
Yucel K, McKinnon MC, Taylor VH, Macdonald K, Alda M, Young LT, MacQueen GM (2007) Bilateral hippocampal volume increases after long-term lithium treatment in patients with bipolar disorder: a longitudinal MRI study. Psychopharmacology 195(3):357–367. https://doi.org/10.1007/s00213-007-0906-9
Yushkevich PA, Amaral RSC, Augustinack JC, Bender AR, Bernstein JD, Boccardi M, Bocchetta M, Burggren AC, Carr VA, Chakravarty MM, Chételat G, Daugherty AM, Davachi L, Ding S-L, Ekstrom A, Geerlings MI, Hassan A, Huang Y, Iglesias JE, Zeineh MM (2015) Quantitative comparison of 21 protocols for labeling hippocampal subfields and parahippocampal subregions in in vivo MRI: towards a harmonized segmentation protocol. Neuroimage 111:526–541. https://doi.org/10.1016/j.neuroimage.2015.01.004
Zheng F, Li C, Zhang D, Cui D, Wang Z, Qiu J (2019) Study on the sub-regions volume of hippocampus and amygdala in schizophrenia. Quant Imaging Med Surg 9(6):1025–1036. https://doi.org/10.21037/qims.2019.05.21
Zijdenbos AP, Dawant BM, Margolin RA, Palmer AC (1994) Morphometric analysis of white matter lesions in MR images: method and validation. IEEE Trans Med Imaging 13(4):716–724. https://doi.org/10.1109/42.363096
Zou KH, Warfield SK, Bharatha A, Tempany CMC, Kaus MR, Haker SJ, Wells WM, Jolesz FA, Kikinis R (2004) Statistical validation of image segmentation quality based on a spatial overlap index1. Acad Radiol 11(2):178–189. https://doi.org/10.1016/S1076-6332(03)00671-8
This work was support by internal funds provided by the University of Pittsburgh. In addition, this research used brainlife.io supported with grants from the National Science Foundation (IIS-1912270, IIS-1636893, BCS-1734853) to Dr. Franco Pestilli at The University of Texas at Austin.
The authors have no conflicts of interest to disclose.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1: Table S1
: Intraclass Correlation Coefficients (ICC), Dice Coefficients, Bland–Altman bias as a portion of a volume’s structure (Bias as POV), and Bland-Altman biasranges for Hippocampal Subfields for left and right hemisphere regions (e.g., ICC LH =Intraclass Correlation Coefficients for left hemisphere; Dice RH = Dice Coefficient forright hemisphere). Color coding is in accordance with excellent [green], good [yellow], poor [red] scores for ICCs and Dice Coefficients (ICC: 0.90-1.00 [excellent], 0.75-0.89 [good], 0.00-0.74 [poor]; Dice Coefficients: 0.70-1.00 [excellent], 0.50-.69 [good], 0-0.49 [poor]). We have also highlighted regions with >1% bias as a portion of a volume’s structure in yellow. Subfield Abbreviations include: Cornu Ammonis (CA), Granule Cell and Molecular Layer of Dentate Gyrus (GC-ML-DG); Hippocampus-Amygdala-Transition-Area (HATA); Hippocampal Parcellation (HP). Values were derived using Freesurfer 7 longitudinal processing stream. Table S2: Intraclass Correlation Coefficients (ICCs), Dice Coefficients, Bland–Altman bias as a portion of a volume’s structure (Bias as POV), and Bland-Altman bias ranges for Amygdala Subnuclei for left and right hemisphere regions (e.g.,ICC LH = Intraclass Correlation Coefficients for left hemisphere; Dice RH = Dice Coefficient for right hemisphere). Color coding is in accordance with excellent [green], good [yellow], poor [red] scores for ICCs and Dice Coefficients (ICC: 0.90-1.00 [excellent], 0.75-0.89 [good], 0.00-0.74 [poor]; Dice Coefficients: 0.70-1.00 [excellent], 0.50-.69 [good], 0-0.49 [poor]). We have also highlighted regions with >1% bias as a portion of a volume’s structure in yellow. Values were derived using Freesurfer 7 longitudinal processing stream. Table S3: Correlation coefficient for bivariate correlations between Hippocampal Subfield Dice Coefficients and subject-level covariates: MRI Quality (Difference Score; MRIQ), Sex, and Age. These were completed for left hemisphere (LH) and right hemisphere (RH). Correlations with p<0.05 are highlighted in yellow. Subfield Abbreviations include: Cornu Ammonis (CA), Granule Cell and Molecular Layer of Dentate Gyrus (GC-ML-DG); Hippocampus-Amygdala-Transition-Area (HATA); Hippocampal Parcellation (HP). Values were derived using Freesurfer 7 longitudinal processing stream. Table S4: Correlation coefficient for bivariate correlations between Hippocampal Subfield Dice Coefficients and subject-level covariates: MRI Quality (Difference Score; MRIQ), Sex, and Age. These were completed for left hemisphere (LH) and right hemisphere (RH). Correlations with p<0.05 are highlighted in yellow. Values were derived using Freesurfer 7 longitudinal processing stream. Table S5: Mean and standard deviation (SD) volume estimates across three scans for each Hippocampal Subfield (LH = Left Hemisphere, RH = Right Hemisphere) in millimeters cubed (mm3). Subfield abbreviations include: Cornu Ammonis (CA), Granule Cell and Molecular Layer of Dentate Gyrus (GC-ML-DG); Hippocampus-Amygdala-Transition-Area (HATA); Hippocampal Parcellation (HP). Volume estimates derived using the FreeSurfer 7 longitudinal processing pipeline Table S6: Mean and standard deviation (SD) volume estimates across three scans for Amygdala Subnuclei (LH = Left Hemisphere, RH = Right Hemisphere) in millimeters cubed (mm3). Volume estimates derived using the FreeSurfer 7 longitudinal processing pipeline.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Kahhale, I., Buser, N.J., Madan, C.R. et al. Quantifying numerical and spatial reliability of hippocampal and amygdala subdivisions in FreeSurfer. Brain Inf. 10, 9 (2023). https://doi.org/10.1186/s40708-023-00189-5
- Automated segmentation
- FreeSurfer 7.1