Fig. 1

Stimulus information in mouse auditory cortex during a tone discrimination task. A Mice performed a go/no-go tone discrimination task whilst the activity of A1 L2/3 neurons were recorded with two-photon calcium imaging. In response to a target tone (low-frequency, in orange) mice had to lick a waterspout and not to lick for non-target tones (high-frequency, in blue). Granger causality analysis revealed sparsely connected networks of cells in A1 L2/3 [34]. We classified neurons as GC (purple) and no-GC (black) depending on whether they formed a GC link. B Top: Example of the stimulus information time-course for a single neuron. We computed the time-resolved stimulus information as the mutual information between the auditory stimuli (low-/high-frequency tones) and the spiking activity across trials. Bottom: The traces of the deconvolved spiking activity in each trial, colour-coded based on the tone presented in each trial. The time axes are referenced based on the stimulus onset. C Stimulus information time-course for GC neurons (left map) and no-GC neurons (right map) in correct trials only. We then sorted the peaks of stimulus information for each neuron to tile the trial time. D Stimulus information for GC (in purple) and no-GC (black) neurons computed separately in trials with correct and incorrect choices. Full lines denote the mean across neurons, whilst the shaded areas denote the SEM across neurons