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Brain Informatics

Table 5 Local neuropil subtraction

From: SmaRT2P: a software for generating and processing smart line recording trajectories for population two-photon calcium imaging

  

Anesthetized

Awake

  

Surr = 0

Surr = 1

Surr ≥ 2

Surr = 0

Surr ≥ 1

 

N SLS

1010

330

280

120

160

Local surround

∆ SNR

9.15 ± 5.93

(4e−46)

10.45± 5.49

(6e−8)

– 3.82 ± 0.47

(8e−24)

− 4.19 ± 2.95

(7e−4)

− 1.36 ± 3.82

(4e−3)

∆ pairwise correlations

− 0.337 ± 0.006

(2e−159)

− 0.324 ± 0.011

(9e−54)

− 0.263 ± 0.010

(1e−45)

− 0.313 ± 0.017

(5e−26)

− 0.287 ± 0.018

(2e−19)

PCA

∆ SNR

6.76 ± 0.35

(3e−75)

− 4.28 ± 0.33

(2e−32)

− 5.18 ± 0.43

(8e−28)

− 10.51 ± 2.30

(4e−13)

− 9.98 ± 1.09

(3e−11)

∆ pairwise correlations

− 0.532 ± 0.008

(9e−160)

− 0.495 ± 0.012

(1e−53)

− 0.495 ± 0.013

(1e−45)

− 0.418 ± 0.022

(9e−26)

− 0.448 ± 0.022

(2e−19)

  1. Difference in SNR and pairwise correlations before and after local neuropil subtraction with different methods. Only acquisitions with a reference raster segmentation and a reference box in the SLS trajectory are considered. Mean ± SEM (and p values for Wilcoxon signed rank test)
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