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Table 5 Local neuropil subtraction

From: SmaRT2P: a software for generating and processing smart line recording trajectories for population two-photon calcium imaging

   Anesthetized Awake
   Surr = 0 Surr = 1 Surr ≥ 2 Surr = 0 Surr ≥ 1
  N SLS 1010 330 280 120 160
Local surround ∆ SNR 9.15 ± 5.93
(4e−46)
10.45± 5.49
(6e−8)
– 3.82 ± 0.47
(8e−24)
− 4.19 ± 2.95
(7e−4)
− 1.36 ± 3.82
(4e−3)
∆ pairwise correlations − 0.337 ± 0.006
(2e−159)
− 0.324 ± 0.011
(9e−54)
− 0.263 ± 0.010
(1e−45)
− 0.313 ± 0.017
(5e−26)
− 0.287 ± 0.018
(2e−19)
PCA ∆ SNR 6.76 ± 0.35
(3e−75)
− 4.28 ± 0.33
(2e−32)
− 5.18 ± 0.43
(8e−28)
− 10.51 ± 2.30
(4e−13)
− 9.98 ± 1.09
(3e−11)
∆ pairwise correlations − 0.532 ± 0.008
(9e−160)
− 0.495 ± 0.012
(1e−53)
− 0.495 ± 0.013
(1e−45)
− 0.418 ± 0.022
(9e−26)
− 0.448 ± 0.022
(2e−19)
  1. Difference in SNR and pairwise correlations before and after local neuropil subtraction with different methods. Only acquisitions with a reference raster segmentation and a reference box in the SLS trajectory are considered. Mean ± SEM (and p values for Wilcoxon signed rank test)