Fig. 4

Background activity subtraction. a Top. Fluorescence activity of the ROIs segmented in an example SLS acquisition. Each row in the raster corresponds to the activity of a ROI. Bottom. Fluorescence activity of the same ROIs after the subtraction of the background activity. b Pairwise correlations between ROIs in a before (top) and after (bottom) the subtraction of the background activity. c. Average SNR (blue, left) and pairwise correlations (red, right) difference between background-subtracted and raw data for anesthetized (top) and awake animals (bottom). Extended values in Table 3