Table 1 Milestone innovations of imaging techniques
From: Smart imaging to empower brain-wide neuroscience at single-cell levels
Techniques | Features | Achievements |
|---|---|---|
CLSM [73] | Pin-hole structure to reject out-of-focus light | Improved axial resolution for optical sectioning |
Hundreds of pinholes arranged in spirals on an opaque disk that rotates at high speeds | Vastly speeds up image acquisition and reduce photon damage | |
2P microscopes [76] | Intense excitation by pulsed lasers, leading to absorption of two or three photons at once | Improved light collection efficiency, intrinsic confocal effect, and penetration depth |
Sheet-shaped excitation beam to selectively excite only the plane of interest | Faster imaging procedure, reduced photon damage | |
Simultaneously record multiple views of the specimen | Maximizes the sample coverage | |
A micro-lens array in place of the camera | Capture all voxels in a volumetric image instantaneously |